Vaccine R&D - CGT (Mumbai)

Position: Executive/Senior Executive / Assistant Manager / Deputy Manager – Cell & Gene Therapy (CGT)(Final designation will be aligned with candidate experience)Function / Department:

R&D; – In Vitro CAR-T & Gene TherapyDivision:

Vaccines R&DLocation;:

Genome Valley, Biotech Park, Shameerpet, Hyderabad

Role OverviewThe role involves contributing to preclinical proof-of-concept studies for cutting-edge in vivo cell and gene therapy programs. The incumbent will work on cutting-edge therapies including CAR-T and gene therapy approaches targeting β-thalassemia and sickle cell disease (SCD), supporting translational research and early development.

Key ResponsibilitiesIndependently design, plan, execute, and troubleshoot complex CGT experiments within defined timelines.Analyze, interpret, and present experimental data to support scientific decision-making and project advancement.Collaborate closely with supervisors and cross-functional teams to align on experimental outcomes and future strategies.Support the establishment, operation, and maintenance of mammalian cell culture and CGT laboratories in compliance with quality and regulatory standards.Contribute to process development activities including optimization, scale-up considerations, and technology transfer.Participate in equipment selection, procurement, installation, and qualification for CGT lab operations.Ensure adherence to Good Documentation Practices (GDP), including accurate and timely recording of experimental data in laboratory notebooks or electronic systems.

Qualifications & ExperienceM.Sc.

with 5–10 years of relevant industry/academic experienceORPh.D.

with 3–5 years of postdoctoral or industry experiencePrior experience in Cell & Gene Therapy development is highly preferred

Required Skills & ExpertiseCell Culture & Immunology:Hands-on experience in mammalian cell culture, including primary T-cells, CD34+ stem cells, and cancer cell linesIsolation and culture of immune cells (PBMCs, T-cells, B-cells, NK cells)T-cell proliferation, co-culture systems, cytotoxicity assays, and other immunological assays

Molecular & Gene Editing Techniques:Experience with gene editing tools such as CRISPR, ZFN, TALENTransfection techniques including chemical methods, electroporation, lentiviral vectors, and mRNA-LNPsLentivirus generation, titration, and transductionStable cell line development (Knock-Out, Knock-In, Knock-Down)Analytical & Instrumentation Skills:Multicolor flow cytometry, cell sorting, and Incucyte/live-cell imaging systemsELISA, multiplex assays, Western blottingKnowledge of plasmid mapping, sequence analysis, gene editing validation, and qRT-PCR

Additional ExpectationsStrong problem-solving and analytical capabilitiesAbility to work independently as well as collaboratively in a fast-paced R&D; environmentGood communication and scientific presentation skills Apply on Kit Job: kitjob.in/job/4m0r87